compounds_for_chebi_mapping.txt

# Na2CO3 solution (8% (w/v))
# Vitamin solution
(0.2 ml/10 ml), 1M glucose (0.2 ml/10 ml) and 1M sodium thiosulphate (0.2 ml/10 ml).
(1) CaCl2 2-hydrate
(1) MgSO4 7-hydrate
(1) Micronutrient solution
(1) Salt solution
(10)FeSO4 7-hydrate
(1983)): Agar (Difco) is washed three times for one hour with distilled water at room
(2) CaCl2 2-hydrate
(2) KH2PO4
(2) MgSO4 7-hydrate
(2) Vitamin solution
(3) 2.68 g NH4Cl
(3) MgSO4 7-hydrate
(3) Nutrient solution
(4) CaCl2 2-hydrate
(4) K2HPO4 3-hydrate
(4) MgSO4 7-hydrate
(5) 17.5 g KH2PO4
(5) 2.16 g Na2­ß­glycerophosphate
(5) CuSO4 5-hydrate
(5) EDTAFeNa
(5) FeNa-EDTA
(5) FeNaEDTA
(5) FeSO4 7-hydrate
(5) KH2PO4
(6) CaCl2 2-hydrate
(6) Fe-EDTA:
(7) 75.0 g CaCl2
(7) EDTANa2
(Agar, if necessary
(Agar, if needed
(NH4)2SO4*FeSO4*6H2O,
(NH4)2SO4FeSO4 6-hydrate
(neutralized), vitamins and sucrose from sterile stock solutions.
(using a syringe) 10ml NaHCO3/ 90ml medium and 0.5ml sterile vitamin solution to
* Fe-EDTA complex
*For brackish organisms, use 15 g of NaCl instead of 30 g
*Tryptone
+ 0.02 % Yeast extract
- [ccap_E26_Biotin.md]
- [ccap_E26_Biotin_ANT.md]
.K2HPO4 3-hydrate
.MgSO4 7-hydrate
0.01% NPA
0.01% RPA
0.01g d‐Biotin
0.02g CuSO4 5H2O
0.05 ml/0.10 ml sterile 30% w/v glucose solution
0.05g FeSO4 7H2O
0.1 M K2HPO4 130 ml
0.1 M KH2PO4 70 ml
0.1 M Tris/HCl buffer pH 7.5
0.1% Fe(NH4) citrate solution
0.1% Resazurin
0.1M Tris/HCl at pH 7.5
0.1M Tris/HCl buffer pH 7.5
0.1g p‐aminobenzoic acid
0.2g ZnSO4 7H2O
0.3 mM K2HPO4
0.30 ml/0.60 ml sterile 10% w/v NaHCO3 solution
0.5% MgSO4·7H2O solution
1 M NaHCO3
1% Fe(III) citrate solution
1% Yeast extract
1) 0.5g / litre yeast extract and
1) 1 ml Trace metals solution
1. EDTA
1. NUTRIENT AGAR
1. NUTRIENT AGAR/BROTH
1. Preparation of double layered agar plates:
1.00 g/l glucose at 28°C until stationary phase is reached (7-10 days). Centrifuge culture
1.5% washed agar
10% NaHCO3 (medium is turning from yellow to pink).
10% cysteine
10% glucose
100x Vitamin solution
101. NUTRIENT AGAR or BROTH WITH NaCl
1026. XED-AGAR
1027. YEAST STARCH AGAR
1039. ANACKER-ORDAL AGAR
1061. MODIFIED TH AGAR
1075. PPES-II AGAR MEDIUM
1076a. SP4 MEDIUM WITH GLUCOSE
1081a. HORIKOSHI-1 MEDIUM WITH 10% NaCl
1087. MANNITOL-YEAST EXTRACT-PEPTONE
118. DAP-NUTRIENT AGAR
1190: FERROUS SULFATE/YEAST EXTRACT MEDIUM
12.0 g/l agar.
1203. FASTIDIOUS ANAEROBE AGAR
121°C for 20 min. Grow prey bacterium (DSM 15416) on agar plates using medium 514.
123. MARINE AGAR
1240. STARCH-MINERAL SALT-AGAR + 10% NACL
1241. STARCH - MINERAL SALT - AGAR (STMS) + 15% NaCl
1258. NUTRIMENT AGAR WITH 150 G/L NACL
1259. NUTRIMENT AGAR
1261. ISP 2 MEDIUM WITH 10% NACL
1262. CM + YE MEDIUM + 10% NACL
1274. YEAST EXTRACT-STARCH AGAR
129. POTATO DEXTROSE AGAR
130. CZAPEK-DOX AGAR
1314. YEAST EXTRACT-STARCH AGAR
1317. MODIFIED NUTRIENT AGAR
1331. COLUMBIA AGAR WITH 5% SHEEP BLOOD
1350. TRYPTICASE SOY AGAR NA-MG
1365a. 1/10 R2A MEDIUM MODIFIED
1425. SSE/HD 1:10 MEDIUM
1471. PEPTONE BROTH
1472. MODIFIED TRYPTONE SOYA BROTH
1480. MODIFIED TRYPTONE SOYA BROTH
1484. Glycerol-Yeast extract-Peptone Medium
1489. TSB-GLUCOSE MEDIUM
15.0 g/l agar.
1509. NB-PYRUVATE AGAR
1530. MODIFIED TRYPTONE SOY BROTH/AGAR
1535. MEDIUM D
1559. TRYPTONE-STARCH MEDIUM
1609. HEART INFUSION BLOOD AGAR
1682. Yeast nitrogen base glucose
1691. Artificial sea water – Tryptone soy broth Medium
170. YEAST EXTRACT - SKIM MILK MEDIUM
1716. DILUTED TRYPTONE SOYA BROTH (TSB) 1:10
1725. TRYPTONE-YEAST EXTRACT MEDIUM modified
1749. Sucrose-Asparagine Medium
191. CORN MEAL AGAR
1: Acidic thiamine / Ca pantothenate solution
1M MgCl2
1M Tris-HCl
2 H2O and MgCl2 6-hydrate from sterile stock solutions.
2 g/l agar (semi-solid) and dispensing the medium into 10 ml portions in 16 mm
2% FeSO4 7-hydrate
2% NaCl in distilled water
2) 0.5g / litre yeast extract and
2) 1 ml 20% MgSO4 7-hydrate solution
2) Preparation of the “f/2” Trace Metal Working Stock Solution
2,4-Dihydroxybenzoic acid
2. FeSO4 7-hydrate
2.0% (w/v) of Bacto-Agar (Difco).
20 mM MgSO4 7-hydrate
20 mM MgSO4 7-hydrate:
20% Glucose
20.0 g/l agar.
20mM MgSO4 7-hydrate
220. CASO AGAR
220a. REACTIVATION WITH TRYPTONE SOYA BROTH
238. STREPTOMYCIN NUTRIENT AGAR
24264, DSM 24313, DSM 24197 increase the NaCl concentration to 100.0 g/L.
243. SEAWATER YEAST PEPTONE AGAR
245. BLOOD AGAR II
246. SEA WATER AGAR
248. OAT FLAKES AGAR WITH YEAST EXTRACT
25 ml solution 1 (4 g NaCl, 0.2 g KCl, 0.05 g MgSO4 7-hydrate, 0.05 g MgCl2 6-hydrate
250. PEPTONE MEAT EXTRACT GLYCEROL AGAR
251. PEPTONE - MEAT EXTRACT - SOIL EXTRACT AGAR
252. STARCH - MINERAL SALT - AGAR
258. NUTRIENT STARCH AGAR
262. J - AGAR
277. CHERRY AGAR
2: Basic vitamin solution
3% (w/v) cysteine-HCl x H2O
3% NaHCO3
3) Preparation of the Vitamin Working Stock Solution
3. ZnSO4 7-hydrate
30 mM CaCl2 2-hydrate:
306. NY-AGAR
309. NEOMYCIN AGAR
30mM CaCl2 2-hydrate
31. ALKALINE NUTRIENT AGAR
310. V-8 JUICE AGAR
353. TOMATO JUICE MILK AGAR
39. PFENNIG'S MEDIUM I WITH YEAST EXTRACT
3: Neutral folic acid / riboflavin solution
4) 1 ml 0.01 % Vitamin B12 solution
420. BLOOD AGAR I
425. OATMEAL AGAR
429 e. COLUMBIA BLOOD AGAR 10%
429. COLUMBIA BLOOD AGAR
429a. COLUMBIA BLOOD AGAR WITH CHARCOAL
429b. CHOCOLATE AGAR
429c. COLUMBIA-GLUCOSE-CYSTEINE-AGAR
440. SKIM MILK-HORNMEAL-MINERAL AGAR
457c. MEDIUM WITH CHLOROACRYLIC ACID
459. SUCROSE-PEPTONE-MEDIUM
463a. EDTA-MEDIUM
464. PLATE COUNT AGAR
473. MEDIUM WITH EDTA AS CARBON SOURCE
474. MEDIUM WITH POLYHYDROXYBUTYRIC ACID
48: TRYPTONE THIOGLYCOLATE MEDIUM
4: Neutral B12 solution
5 mM CaCl2 2-hydrate
5% (w/v) NaHCO3
5) 10 ml of 1M Isobutyroamide (8.7 g in 100 ml H2O dest) solution
50x Heterotrophic basal salts solution
525a: METHANOHALOPHILUS (MG+NaCl) MEDIUM
53. CORYNEBACTERIUM AGAR
535. TRYPTICASE SOY BROTH AGAR
535. TRYPTONE SOYA AGAR
535a. TRYPTICASE SOY BROTH AGAR+BLOOD
535c. TRYPTICASE SOY BROTH AGAR
54. GLUCOSE YEAST EXTRACT AGAR
545. TRYPTONE SOYA BROTH
545: TRYPTONE SOYA BROTH
545a. DILUTED TRYPTONE SOYA BROTH
548. BENNETT'S AGAR
551. EMERSON'S YEAST STARCH AGAR
555. SPORULATION-AGAR
56. TRYPTICASE STARCH AGAR
578. TRYPTONE-YEAST EXTRACT MEDIUM
581. GLYCEROL CORNSTEEP AGAR
584. BRUCELLA AGAR
585. BCYE AGAR
5g / litre glucose
6-hydrate and glucose from sterile, anaerobic stock solutions.
604. BACTO MARINE AGAR
605. NUTRIENT AGAR
605a. NUTRIENT AGAR (OXOID CM3) WITH PHOSPHATE
609. OATMEAL AGAR (A)
617a. BLOOD AGAR BASE
621. PYGV AGAR
637. STANDARD I + MALT AGAR
646. RABBIT BLOOD AGAR
653. PEPTONE-SUCCINATE AGAR
656. YEAST-STARCH-AGAR
657. COW MANURE AGAR
658. HICKEY-TRESNER-AGAR
67. CY-AGAR
672. HALF STRENGTH NUTRIENT BROTH OR AGAR
680. TRYPTONE YEAST EXTRACT AGAR
687. COLUMBIA BLOOD AGAR
7-vitamin solution
715. COLUMBIA AGAR
729. MYX AGAR
735. MALT EXTRACT GLUCOSE AGAR
781. DUNG EXTRACT AGAR
790. PEPTONE-YEAST MEDIUM with MgSO4
8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v), if necessary.
80. GLYCEROL-SOIL MEDIUM
810. SKIM MILK/GLUCOSE AGAR
82. BHI-GLUCOSE MEDIUM
83. CZAPEK PEPTONE AGAR
830 first. Subsequent subcultivation may be carried out in liquid medium or on agar plates.
852. GYP GLUCOSE-YEAST-PEPTONE MEDIUM
884. TWEEN 80-AGAR
893. TS AGAR
9. VY/2 AGAR
90. MALT EXTRACT PEPTONE AGAR
907. PSEUDOMONAS AGAR F
91. PROPIONIBACTERIUM AGAR
911. MIDDLEBROCK 7H11 AGAR
917. MODIFIED SEA WATER AGAR
924a: METHANOFOLLIS MB MEDIUM
924b: METHANOSARCINA MB MEDIUM
938. ST21 AGAR FOR CELLULOSE DEGRADERS
942. AGAR WITH E. COLI AS FEED
952. 1/10 NUTRIENT AGAR NO.2
953. 1/100 NUTRIENT AGAR NO.2
958. VY/4-SWS AGAR
984. NL 333-AGAR MEDIUM
988. YEAST-PEPTONE-SUCCINATE MEDIUM
993c. MODIFIED ISP5 MEDIUM WITH SODIUM CHLORIDE
Acetate or capronate solution
Agar (BD Bacto), for semi-solid medium
Agar (BD Bacto), for solid medium
Agar (Bacto), for solid medium
Agar (Difco) if appropriate
Agar (Difco), if necessary
Agar (Oxoid No. 1) if appropriate
Agar (if required)………………..15g
Agar Difco
Agar Noble……………..…………10.0 g
Agar agar
Agar if appropriate
Agar if required
Agar, for solid medium
Agar, for stabs
Agar, if necessary
Agar, if needed
Agar, if required
Agar-Agar
Agar-agar
AlCl3 6-hydrate
Allen's trace element solution
Allochromatium
Alternatively
Amino acid solution
Aromatic fatty acids solution
Artificial Seawater suppl. with 50 g/I NaCl1)
Ascorbic acid solution
BG110
BaCl2 2-hydrate
Bacteriological Agar (Oxoid L11) *
Bacteriological agar
Bacto Agar
Bacto Bitone
Bacto Middelbrook 7H11 agar
Bacto Middelbrook 7H11 agar (Difco 283810):
Bacto Middlebrook 7H10 agar
Bacto Middlebrook 7H10 agar:
Bacto Panton
Bacto Peptone
Bacto Proteose Peptone
Bacto Tryptone
Bacto Yeast Extract
Bacto Yeast extract
Bacto agar
Bacto beef extract
Bacto peptone
Bacto yeast extract
Bacto-Peptone
Bacto-Tryptone
Bacto-peptone
BactoAgar
Bactopeptone
Basal salts solution
Beef Extract
Beef extract
Betaine Hydrochloride
Bicarbonate
Biotin solution
Boil medium under CO2 until the resazurin indicator changes from pink to colourless.
Branched
Branched-chain fatty acids solution
C6H9NO6
CCAP
CII
CMA
CYE-ACES AGAR
Ca2Cl 2-hydrate
CaC03
CaCL2 2-hydrate
CaCl 2-hydrate
CaCl2 * 2H2O
CaCl2 1.5%
CaCl2 2-hydrate
CaCl2 2-hydrate solution
CaCl2 2-hydrate…………………….0.5 g
CaCl2 6-hydrate
CaCl2 7-hydrate
CaCl2 x 2 H20
CaCl2 x H20
CaCl2 x H2O
CaSO4 2-hydrate
Carbon source solution
Carbonate Salts Solution
Casamino Acid
Casamino acid
Casein acids hydrolysate
Casein digest (Casitone, cat. no. 225930) *
Casein hydrolysate
Casein hydrolysate acid
Casein peptone
Casein peptone, tryptic digest
Casein peptone, tryptic digested
Casitone
Casmino Acid
CeCl3 7-hydrate
Charcoal for medical application
Co(NO3)2 6-hydrate
Co-factors solution
CoCL2 6-hydrate
CoCl2 2-hydrate
CoCl2 4-hydrate
CoCl2 6 H2O
CoCl2 6-hydrate
CoSO4 7-hydrate
Columbia Blood Agar Base
Combine
Combine 16.04 ml of 0.5 M K2HPO4 with 3.96 ml of 0.5 M KH2PO4 stock solution to reach a
Corn meal Agar
Corn meal agar
Cresol red solution
CuCl2 2-hydrate
CuCl2 5-hydrate
CuCl2 6-hydrate
CuCl2 x H2O
CuSO2 5-hydrate
CuSO4 2-hydrate
CuSO4 4-hydrate
CuSO4 5-hydrate
CuSO4 5-hydrate……………………0.079 g
CuSO4 6-hydrate
CuSO4 x H2O
CuSo4 5-hydrate
Cultures of myxobacteria delivered as active cultures (growing on agar plates): Always
Cysteine - HCl x H2O
Cysteine HCl
Cysteine and Na2S solutions:
Cysteine hydrochloride solution
Cysteine hydrochloride solution (10%):
Cysteine solution
Cysteine-HCL x H2O
Cysteine-HCl
Cysteine-HCl x H2O
Czapek Dox agar
D-fructose as substrate.
DSM 104091 may be grown in the presence of 0.5% glucose instead of cellulose.
DSM 111909 may be grown on 1.0% methanol and for solid media use 15g agar per
DSM 16139 and DSM 16140 have been grown in medium containing 45 g/l NaCl.
Distilled water to
Dithiothreitol, 100 mM solution
Don't use Difco Bacto-peptone, it was reported in 1988 to contain bile salts that lyse
Dung extract
E26 + biotin
E26 + biotin + ANT
EDTA 2-hydrate
EDTA Na2
EDTA Solution
EDTA-Na2
EDTA-iron
EDTA.Na2 2-hydrate
EDTANa2
EDTA………………………………0.5 mg
Enzymatic casein hydrolysate
Enzymatic digest of casein
Enzymatic hydrolysate of casein
FWM stock solution
Fastidious Anaerobe Agar
Fe EDTA
Fe(III) citrate solution
Fe(III)NH4-EDTA
Fe-EDTA
Fe-EDTA complex *
Fe-NH4-citrate
Fe-NH4EDTA
Fe3Cl 6-hydrate
FeCL2 4-hydrate
FeCL3 6-hydrate
FeCl2 4-hydrate
FeCl2 6-hydrate
FeCl2 7-hydrate
FeCl3 4-hydrate
FeCl3 6-hydrate
FeEDTA
FeEDTA solution
FeEDTA solution:
FeNa-EDTA
FeNa-EDTA:
FeNaEDTA
FeSO2 7-hydrate
FeSO4 2-hydrate
FeSO4 6-hydrate
FeSO4 7-hydrate
FeSO4 7-hydrate (10 mM) in HCl
FeSO4 7-hydrate solution
FeSO4 7-hydrate solution (0.1% w/v)
FeSO4 solution see below
FeSO4 solution:
FeSO4 x 7 H20
FeSO4(NH4)2 6-hydrate
Ferric EDTA solution
Ferric EDTA solution:
Fe‐NH4‐citrate
Filtered natural seawater
Folic acid solution
For Allochromatium phaeobacterium DSM 19781 reduce the amount of NaCl to 10.0 g/l
For agar plates increase the pH of the Tris/HCl buffer to 8.5.
For soft agar for the maintenance of active cultures reduce agar content to 4.0 g/l.
For solid medium use 15.0 g agar.
Formiate/Fumarate Solution
Fresh yeast extract
Freshwater Solution
Fructose 0.480 g
GYS
GYSW
Glacial acetic acid
Glucose 0.480 g
Glucose 10%
Glucose 3g/l.
Glucose 50 % solution
Glucose minerals salts medium
Glucose or Mannitol
Glutamine
Glutathion
Glutathione solution
Growth with thiosulfate (40 mM) and NH4Cl (5 mM); in conical flasks 1/10 liquid/air ratio
H3BO
H3BO2
H3BO4
HALOACTINOMYCES
HBO3
HEPES buffer solution
HEPES buffer solution:
Haemin solution
Hemin solution
III
IRD
If required, Agar-agar
Increase
Increase the NH4Cl to 0.68 g/l
It is strongly suggested that Bacto peptone (BD 212750) and Bacto yeast extract (BD
K2CrO4
K2HPO4 * 3H2O
K2HPO4 3-hydrate
K2HPO4 3-hydrate………………….0.75 g
K2S4O6
KCl………………………………....0.5 g
KH2PO4
KH2PO4 7-hydrate
KH2PO4, 1.5 g and K2HPO4, 0.5 g.
KNO2
L-Cysteine HCl
L-Cysteine HCl x H2O
L-Cysteine x HCL
L-Cysteine x HCl
L-Cysteine-HCl
L-Cysteine-HCl x H2O
L-cysteine HCl
L-cysteine HCl from sterile stock solutions.
L-cysteine-HCl x H2O
LIP-solution
LMG
LaCl3 7-hydrate
Lab-Lemco beef extract
Li2B4O7
Lipoic acid solution
Liquefy agar by steaming at 100C for 15-20 minutes.
MARINE AGAR 2216 (Difco 0979).
MBBM
MC (Modified Chang's Serum­Casein­Glucose­Yeast
METHANOFOLLIS
METHANOSARCINA
MODIFIED
MSC
MY75S
Maize extract
Malt extract
Marine trace elements solution
Meat Extract
Meat extract
Meat peptone
Medium may be solidified with 15.0 g/l agar. pH 7.0
Menadione solution
Mg(SO4) 7-hydrate
Mg-citrate
Mg2SO4
MgCL2 6-hydrate
MgCl 6-hydrate
MgCl 7-hydrate
MgCl2 * 6H2O
MgCl2 2-hydrate
MgCl2 4-hydrate
MgCl2 6-hydrate
MgCl2 6-hydrate……………………2.0 g
MgCl2 7-hydrate
MgCl2 76-hydrate
MgCl2 X 6H2O
MgCl2 solution before pouring the plates.
MgCl2 x 6 H20
MgCl2 x 6H20
MgCl2 x H2O
MgCl2X6H2O
MgSO 7-hydrate
MgSO2 7-hydrate
MgSO4 * 7H2O
MgSO4 6-hydrate
MgSO4 7-hydrate
MgSO4 7-hydrate 0.50
MgSO4 7-hydrate as sterile solutions.
MgSO4 7-hydrate solution
MgSO4 7-hydrate to 15.0 g).
MgSO4 7-hydrate…………………..3.5 g
MgSO4 x 7 H20
MgSO4 x 7H2
MgSO4 x 7H20
MgSo4 7-hydrate
Mineral salt solution
Mineral solution
Minerals solution
MnCl
MnCl2 2-hydrate
MnCl2 4-hydrate
MnSO4 1-hydrate
MnSO4 2-hydrate
MnSO4 4-hydrate
MnSO4 5-hydrate
MnSO4 6-hydrate
MnSO4 7-hydrate
MnSO4 x H2O
MnSO4x H2O
Modified Wolin's mineral solution
N-Z amine A
N-acetylglucosamin- and pyruvate solution
NH4 (III)citrate
NH4Cl -
NH4H2PO4
NH4HCO3
NH4MoO4
NH4NO3
NH4SO4
NN (Non-Nutrient (Amoeba Saline) Agar)
NN (Non­Nutrient (Amoeba Saline) Agar)
NPB agar plate, colony formation is observed after 3-4 days of incubation at 30C.
NTA x H2O
NZ Case (Wako pure chemicals, Japan)=Peptone from Casein 3.000
NZ Case (Wako pure chemicals, Japan)=Peptone from Caseine 3.000
Na Acetate
Na acetate
Na-Ascorbate solution
Na-Caseinate
Na-EDTA 2-hydrate
Na-caseinate
Na-citrate
Na-glycerol phosphate
Na-glycerophosphate
Na-malate or –acetate
Na-resazurin solution
Na-thiosulfate, 5% w/v solution
Na2 x EDTA
Na2-EDTA 2-hydrate
Na2-citrate
Na2-ß-glycerolphosphate
Na2B4O7
Na2B4O7 10-hydrate
Na2CO3 anhydrous
Na2CO3 solution:
Na2CO3, and NaHCO3 using anaerobic conditions, under nitrogen. If the medium has been
Na2CO3, if necessary.
Na2CO3…………………………...0.02 g
Na2EDTA 2-hydrate
Na2H2PO4 x H2O
Na2HAsO4 7-hydrate
Na2HPO4 12-hydrate
Na2HPO4 2-hydrate
Na2HPO4 7-hydrate
Na2HPO4 x H2O
Na2Mo4 2-hydrate
Na2MoO4 2-hydrate
Na2MoO4 7-hydrate
Na2MoO4 x H2O
Na2MoO7O4 2-hydrate
Na2MoSO4 2-hydrate
Na2O4W 2-hydrate
Na2S 9 H2O
Na2S 9-hydrate
Na2S solution
Na2S-cysteine solution
Na2S2O3 5-hydrate
Na2S2O3 x 5 H20 and NaHCO3 in serum bottles and seal the serum tubes with butyl
Na2S2O4
Na2SO4 10-hydrate
Na2Se2O3
Na2Se3 5-hydrate
Na2SeO3 5 H2O
Na2SeO3 5-hydrate
Na2SeO4
Na2SeO4 10-hydrate
Na2SiO3 9-hydrate
Na2SxH2O
Na2WO4 2-hydrate
Na2WoO4 2-hydrate
Na2‐EDTA * 2H2O
Na2‐EDTA 2-hydrate
Na3-Citrate
Na3-citrate
NaCH3COO
NaCO3
NaCl 100 - 150.00
NaCl 20-25 %
NaCl 5%
NaClO3
NaCl……………………………….25.0 g
NaH2PO4 2-hydrate
NaH2PO4 x H2O
NaHCO3 (10%; w/v) in distilled water, store at 4°C
NaHCO3 2.625 g per 50 ml distilled water
NaHCO3 solution
NaHCO3, 5% w/v solution
NaHSeO3
NaMoO4 2-hydrate
NaNH4HPO4 4-hydrate
NaNO3
NaS2O3
NaS2O3 5-hydrate
NaSeO3
NaSiO3 9-hydrate
NaVO3 x H2O
NaWO4 6-hydrate
Naphthalene sulfonic acid
NdCl3 6-hydrate
Neopeptone
Ni2SO4 6-hydrate
NiCL2 6-hydrate
NiCl2 2-hydrate
NiCl2 6-hydrate
NiSO4 6-hydrate
Nitsch Element Solution
Nutrient Agar
Nutrient agar (Oxoid CM3)*
OTEB is a complex peptone-yeast extract medium containing volatile fatty acids and serum for a
Or "Ultramarine" Synthetica sea salts*
Or "Ultramarine" Synthetica sea salts**
PPG
PPY
Pancreatic Digest of Casein
Pancreatic digest of casein
Peptone
Peptone bacteriological
Peptone from Casein
Peptone from casein
Peptone from caseine
Peptone from meat
Peptone from soy meal
Peptone from soybean
Peptone from soybeans
Peptone from soymeal
Peptone protease
Peptone, casein digest
Peptone-yeast-glucose medium
Phosphate solution
Phytone peptone
Polypeptone
PrCl3 x H2O
Primary Stock Solution
Proteose Peptone
Proteose Peptone #2
Proteose Peptone BD
Proteose Peptone No. 3
Proteose Peptone n°3
Proteose peptone
Proteose peptone No 2
Proteose peptone No. 3
Proteose peptone Nr. 3
Proteose peptone no. 3
Proteose peptone no.3
Proteose tryptone
Purified agar
Pyromelitic acid
RbCl
Reduce the MgSO4 7-hydrate to 1.00 g/l
Reduce the MgSO4 7-hydrate to 1.5 g/l
Rehydrate and grow lyophilized cells from the ampoule in liquid tryptone soya broth (see
Resazurin 0.025%
Resazurin solution
Resazurin solution:
Riboflavin solution
SABOURAUD-2% Glucose-Bouillon
SAF
SIGMA
SII
SL-6 trace element solution
SL10
SNA
SNA/5
SODIUM CHLORIDE
Salt Water Solution
Salt solution
Same as solutions A and B of medium 585, CYE-ACES agar.
Se-acid
Selenite and tungstate solution
Selenite-Tungstate solution
Selenite-tungstate solution
Selenite-tungstate-molybdate solution
Selenite/tungstate solution
Seven vitamins solution
SnCl2 2-hydrate
Sodium Chloride
Sodium carbonate solution
Sodium resazurin
Soil extract
Soja peptone
Solidify
Solidify with 5g/l agar
Solution
Solution 2 (100 mM Fe EDTA):
Solution 2 (Fe EDTA):
Solution 2 NaHCO3
Solution 3 (100 mM KH2PO4):
Solution 4 (10 mM CuSO4 5-hydrate):
Solution 5
Solution 5 (30% NaCl):
Solution 6 (100 mM NH4Cl):
Solution 6 (Fe EDTA))
Solution 6 (Fe EDTA):
Solution 8 (100 mM Hepes):
Sorbit or D-Glucose
Soy peptone
Soya peptone
SrCl2 6-hydrate
Stock solution
Stock solution 6
Subsequent subcultivation may be carried out in liquid medium or on agar plates, or in
Subsequent subculturing may be carried out in liquid medium 545 or on agar plates.
Subsequent subculturing may be carried out in liquid medium or on agar plates medium
Subsequent subculturing may be carried out in liquid medium or on agar plates.
Subsequent subculturing may be carried out on agar plates also.
Subsequently subculturing may be carried out in liquid medium or an agar medium.
Substitute
Substitute 1.0 g/l MgSO4 7-hydrate for the MgCl2 6-hydrate
Substrate
Sucrose Solution
Sucrose-Phosphate-Glutamate buffer
Sugar Stock Solution
Sugar solution
TAPSO
TRACE ELEMENT SOLUTION
TYC-solution
Tetramethyl ammonium chloride
The medium may be solidified with 15 g/l agar
The medium may be solifidied with 17 g/l agar.
The original medium description specifies polypeptone, which appears to be no longer
The pH of the completed medium should be 6.8 - 7.0. For growth an agar plates,
The strains DSM xxxx only grow on agar based media and not in liquid media. The gas
The vitamin solution, trace element solution, Se/W solution, cysteine, glucose, Na2S and
Thiamine-HCl 2-hydrate
Thiamine-HCl-2H2O
Thoroughly disperse the agar in cold amoeba saline solution and then slowly bring
Titanium citrate solution
To medium 514 increase the Bacto peptone concentration to 10 g/l, the Bacto yeast
Trace Element Solution
Trace element solution
Trace elements solution
Trace metals solution
Trace mineral salts solution
Trace salt solution
Traceelements solution
Tri-Na citrate
Tri-sodium citrate
Tris-HCl buffer
Tris-HCl buffer, 0.1 M, pH 7.5
Tris/HCl-buffer 10mM, pH 7.8
Trypt. Peptone
Trypticase Peptone
Trypticase Soy Agar (BBL 11043) with 5% defibrinated sheep blood or Columbia Agar
Trypticase Soy Broth with Yeast Extract
Trypticase peptone
Tryptone
Tryptone Peptone
Tryptone Soy Broth
Tryptone peptone
Tryptose-phosphate
Universal peptone
Use 30 mM thiosulphate and up to a maximum of 5 mM NH4Cl
Use 40 mM thiosulphate, 5 mM KNO3, reduce the amount of MgCl2 to 0.5 mM
Use 80 mM thiosulphate, 5 mM KNO3, reduce the amount of MgCl2 to 0.5 mM
Use medium 480 without yeast extract, sodium acetate and NaHCO3.
Use medium 607 with modification peptone, yeast extract and glucose in triple
Use medium 711 with 1g/l yeast extract, without acetate, and with 5 g/l fructose as the
Use medium 756 with 2 g/l NaNO2, but use 0.15 g/l yeast extract, 0.15 g/l peptone, and
VOSO4 2-hydrate
VOSO4 5-hydrate
VOSO4 x H2O
Vitamin B1 solution
Vitamin B12 solution
Vitamin K1 solution
Vitamin K3 solution
Vitamin Solution
Vitamin Stock solution
Vitamin solution
Vitamine Solution
VoSO4 2-hydrate
Volatile fatty acid solution
WITH
WITH CYANURIC ACID
WITH CYSTEINE
WITH MODIFIED NACL CONCENTRATION
WITH NaCl
WITH PYRROLIC ACID
Waris-H Reduced + Soil Extract
We recommend that liquid and solid media be made by autoclaving the Na2CO3
Wolfe’s Mineral Solution
Wolin's vitamin solution
Working Stock Solution
XCF
Yeast Extract
Yeast extract
Yeast extract Oxoid
Yeast extract solution
Yeast extract, 10% w/v solution
Yeast extract, and 15 g agar. Alternatively medium 514 may be used.
Yeast extract, dehydrated
ZM/10
ZnClSO4 7-hydrate
ZnSO4 6-hydrate
ZnSO4 7-hydrate
ZnSO4 7-hydrate…………………….0.222 g
about 55C otherwise hydrolysis of the agar may occur.
agar maker is preferable to making this medium. The constant stirring during
agar or 12 g/l of agarose (Sigma).
agar tubes.
agar-medium.
allowed to solidify. A thin layer (5-10 ml) of the growth medium containing 1% agar is
amount of yeast extract to 1 g/l.
amount of yeast extract to 2.00 g/l.
amounts of Na2S 9-hydrate, cellulobiose and yeast extract from sterile, anaerobic stock
amounts of Na2S 9-hydrate, vitamin solution, Selenite-tungstate solution and glucose from
and N-Z amine by Bacto casein (2.0 g, Difco).
and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers.
and NaHCO3 using anaerobic conditions, under nitrogen. If the medium has been boiled
and agar forms.
and increase the amount of NH4Cl to 0.64 g/l.
and the glucose or N-acetylglucosamine (if used as substrate) from sterile stock solution
anoxic stock solution of 5% (w/v) Na2CO3, if necessary.
autoclaving
autoclaving 1.00 g/l yeast extract and 2.50 g/l Na-L-Lactate from sterile anoxic stock
beginning
biotin or 1.0 g/l of yeast extract.
broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates.
broth. Subsequent subculturing may be carried out in liquid medium or on agar plates
broth. Subsequent subculturing may be carried out in liquid medium or on agar plates.
calcium chloride, glucose, vitamins, cysteine and sulfide from sterile anoxic stock solutions
chloride, calcium chloride, vitamins, glucose and sulfide from sterile anoxic stock solutions
chloride, serine, vitamins, cysteine, and sulfide from sterile anoxic stock solutions
chloride, trace elements, yeast extract, pyruvate, arsenate, vitamins and sulfide from
citrate 2-hydrate
components at 60°C before pouring the agar medium.
concentration
contact with the fresh agar plate.
containing
containing 457-agar medium.
cultures
cysteine solutions.
di- or trisodium salt
di-Na-ß-glycerophosphate
dispensing
elements and agar.
elements, yeast extract, formate, pyruvate, thiosulfate, vitamins and sulfide from sterile
elements, yeast extract, lactate, sulfite, vitamins, and sulfide from sterile anoxic stock
extract
extract, vitamins, cysteine and ferric iron hydroxide sludge. Suspend pellet of ferric iron
filtration
filtration), trimethylamine, cysteine and sulfide from sterile anoxic stock solutions
filtration. Increase amount of yeast extract to 2.00 g/l.
first. Subsequent subcultivation may be carried out in liquid medium or on agar plates and
g/l D-glucose from sterile anoxic stock solutions.
g/l agar.
g/l yeast extract.
g/l yeast extract; anaerobic
g/l), NaHCO3 solution (100 g/l), Na2S solution (100 g/l); pH 7.5) and trace vitamin
glucose and yeast extract. Bring contents to boil. Transfer molten agar to suitable vessels.
glucose, maltose, cellobiose, starch, cysteine and sulfide from sterile anoxic stock
glutamine
growth after ten days, carefully split up the agar-culture-cubes and squeeze the material
hydrogenphosphates, creatinine, vitamins, cysteine and sulfide from sterile anoxic stock
in one medium, arginine can inhibit the growth of some glucose utilizers (unknown
increase
lancet. Make sure that the pieces of swarm colonies grown on the agar are transferred to
layer of growth medium containing 0.5% agar (about 35 ml) is poured into a plate and
liquid
magnesium sulfate, calcium chloride, yeast extract and glucose from sterile stock
maintenance
medium
medium 545). Subsequent subculturing may be carried out in liquid medium or on agar
medium to 7.6 with a sterile 5% (w/v) Na2CO3 solution.
medium to 7.8 using a sterile anoxic stock solution of 5% (w/v) Na2CO3.
methanol (50% v/v solution), cysteine and sulfide from sterile anoxic stock solutions
min. For agar slants use 15.0 g agar per 1000.0 ml medium.
ml of a sterile anoxic stock solution of MgCl2 6-hydrate (40% w/v). Store the indole-salt
not grow on plates containing 1-2% agar, and a double layer system has to be used. A
of the swarm and place onto a fresh agar plate using an appropriate tool such as a
of yeast extract to 2.00 g/l.
one 24 h old agar slant culture and wash off grown cells in 0.5 ml sterile water.
one CaCl2-agar plate and streak every drop once to receive E. coli lines, about 3 cm long,
out the agar.
pancreatic digest of casein
peptone
peptone (BD BBL) as substrates.
periodically with sterile yeast extract solution (0.1% end concentration, for more details
precipitate
preparation of solidified agar medium.
reduce amount of yeast extract to 0.10 g/l.
resazurin and cysteine.
serves as a buffer. The calcium carbonate will settle in agar plates before the agar has
settle in agar plates before the agar has set, producing an opaque layer in the bottom.
solidified
solifidied
solution
solution (see medium 503), 2.50 g/l Na-pyruvate and 0.50 g/l yeast extract from anoxic
solution of Na2CO3 (5% w/v).
solutions (10% Na2S 9-hydrate, 5% cellulobiose, 10% yeast extract).
soya peptone
sterile stock solutions the CaCl2, MgCl2, KNO3, tryptone, yeast extract, vitamins and
sterilised stock solution and the NaHCO3, Na2S 9-hydrate, Na2S2O3 5-hydrate from sterile
sterilised under N2), histidine, yeast extract, and Na2S 9-hydrate from anaerobic, sterile
stock solution
subculturing may be carried out in liquid medium or on agar plates, on medium 830, 464
subculturing may be carried out in liquid medium or on agar plates.
subculturing may be carried out in liquid medium or on agar.
sucrose. The CaCl2, MgCl2, KNO3, tryptone, yeast extract, and sucrose stock solutions
supplied the information that the strain grows better on a peptone originally available in
surface, spread about 1 ml of sterile medium or water over the agar plates and briefly
swarm adheres to the agar or grows within the agar, cut small agar cubes from the rim
the NaCl.
the agar plate. Attempt to place the inoculum in such a way that the swarms are in
the agar will darken and degrade.
the surface of agarized medium 67.
thiosulfate, yeast extract, Tryptone, Casamino acids and sulfide from sterile anoxic stock
through
to 6.8-7.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v), if necessary.
to yellow. For stock cultures, 5 g l-1 CaCO3 (will stay as a precipitate) or 4.8 g l-1 HEPES
trace elements, glycerol, vitamins, and sulfide from a sterile anoxic stock solution
transfer high amounts of cell mass to several spots on fresh VY/2 agar medium. If the
transfer onto agar plates.
under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution
under 80% N2 and 20% CO2 gas atmosphere and yeast extract, vitamins and sulfide from
under N2), sucrose, yeast extract, and Na2S 9-hydrate from anaerobic, sterile stock
use 15 g agar per 1000.0 ml medium.
will settle in agar plates before the agar has set, producing an opaque layer in the
with
with 1 - 2 g washed agar per liter can be used (Widdel et al., Arch. Microbiol. 134:286-294
with 50 g/l NaCl
without glucose
years. Trypsin or EDTA are never used, as the tick cells do not like them.
yeast extract
yeast extract as substrates.
yeast extract to 1.00 g/l.
yeast extract.
yeast extract. When growth has started, feed again same amount of propanol.