This is the data repository for Sivagurunathan et al., 2025. "Alternate dyes for image-based profiling assays." DOI http://doi.org/10.1016/j.slasd.2025.100268
Other data (e.g. images) supporting this publication can be found in the Cell Painting Gallery under prefix cpg0029-chroma-pilot.
Cell Painting, the leading image-based profiling assay, involves staining plated cells with six dyes that mark the different compartments in a cell. Such profiles can then be used to discover connections between samples (whether different cell lines, different genetic treatments, or different compound treatments) as well as to assess particular features impacted by each treatment. Researchers may wish to vary the standard dye panel to assess particular phenotypes, or image cells live while maintaining the ability to cluster profiles overall.